yap1 inhibitor verteporfin  (MedChemExpress)

Journal: Regenerative therapy

Article Title: Transcriptomic analysis of hepatocytes reveals the association between ubiquitin-specific peptidase 1 and yes-associated protein 1 during liver regeneration.

doi: 10.1016/j.reth.2023.07.004

Figure Lengend Snippet: Fig. 1. Elevated hepatic expression of YAP1 during liver regeneration. (A). Histological changes, Ki67 staining, and PCNA staining in pre-PHx and PHx-24 h groups. 10X scale bar: 250 mm, 40X scale bar: 50 mm. (BeC). Protein expression levels of YAP1 in pre-PHx and PHx-24 h groups. ***P < 0.001. (D). Serum levels of ALB in pre-PHx and PHx-24 h groups. ***P < 0.001. (E). Immunocytofluorescense staining of hepatic ALB in pre-PHx and PHx-24 h groups. pre-PHx: before partial hepatectomy, PHx-24 h: 24 h post partial hepatectomy, HE: hematoxylin-eosin staining, PCNA: proliferating cell nuclear antigen, YAP1: yes-associated protein 1, ALB: albumin.

Article Snippet: Verteporfin, a YAP1 inhibitor, was acquired from Selleck (Selleck, USA).

Techniques: Expressing, Staining

Journal: Regenerative therapy

Article Title: Transcriptomic analysis of hepatocytes reveals the association between ubiquitin-specific peptidase 1 and yes-associated protein 1 during liver regeneration.

doi: 10.1016/j.reth.2023.07.004

Figure Lengend Snippet: Fig. 2. Targeting YAP1 impairs hepatocyte proliferation. (A). Immunocytofluorescense staining of YAP1 in pre-PHx-Hep and PHx-24 h-Hep groups. Enlargement: 40X. (B). Whole, cytoplasma and nucleus expression of YAP1 and PCNA in pre-PHx-Hep and PHx-24 h-Hep groups. (C). siRNA transfection in PHx-24 h-Hep cells. Enlargement: 40X. (DeE). siRNA transfection decreased the expressions of YAP1, Cyclin D1 and PCNA in PHx-24 h-Hep cells. *P < 0.05, **P < 0.01, ***P < 0.001. (FeH). Expression of YAP1 in Vehicle and Verteporfin treated BRL3A cells. **P < 0.01. (GeJ). Expression of Cyclin D1 and PCNA in Vehicle and Verteporfin treated BRL3A cells. *P < 0.05, **P < 0.01. (K). The cell proliferation of BRL3A in Vehicle and Verteporfin treated BRL3A cells. *P < 0.05.

Article Snippet: Verteporfin, a YAP1 inhibitor, was acquired from Selleck (Selleck, USA).

Techniques: Staining, Expressing, Transfection

Journal: Regenerative therapy

Article Title: Transcriptomic analysis of hepatocytes reveals the association between ubiquitin-specific peptidase 1 and yes-associated protein 1 during liver regeneration.

doi: 10.1016/j.reth.2023.07.004

Figure Lengend Snippet: Fig. 3. Identification of the association between USP1 and YAP1 in hepatocyte proliferation. (A). Volcano Plot of differentially expressed genes between the pre-PHx-Hep group and the PHx-24h-Hep group. (B). Heatmap of differentially expressed genes that were up-regulated in the PHx-24h-Hep group. (C). Volcano Plot of differentially expressed genes that were correlated with YAP1 in the liver hepatocellular carcinoma (LIHC) dataset. (D). Heatmap of the top 50 differentially expressed genes that were positively correlated with YAP1 in the LIHC dataset. (E). Venn results showed the common genes between the YAP1 related genes and the DEG1. (F). PPI network of the identified 119 genes, hub genes were showed in red color. (G). Heatmap of the identified hub genes. (H). KEGG analysis of the identified hub genes. (I). Expression of USP1 in pre-PHx-Hep and PHx-24h-Hep groups.

Article Snippet: Verteporfin, a YAP1 inhibitor, was acquired from Selleck (Selleck, USA).

Techniques: Expressing

Journal: Regenerative therapy

Article Title: Transcriptomic analysis of hepatocytes reveals the association between ubiquitin-specific peptidase 1 and yes-associated protein 1 during liver regeneration.

doi: 10.1016/j.reth.2023.07.004

Figure Lengend Snippet: Fig. 4. Overexpression of YAP1 rescues the impairment of hepatocyte proliferation induced by ML-323. (A). GeneMina results showed the interactions among USP1 and YAP1, Cyclin D1, PCNA. (BeD). Correlation analysis between USP1 and YAP1, Cyclin D1, PCNA. (E). Protein expression of YAP1, Cyclin D1 and PCNA in Ctrl and ML-323 treated BRL3A cells. (F). Lenti-virus infection in BRL3A cells with different MOI. Enlargement: 40X. (GeH). Expression of YAP1 in Control and YAP1 overexpressed BRL3A cells. **P < 0.01. (IeK). Protein expression of YAP1 and PCNA in different groups. *P < 0.05, **P < 0.01, ***P < 0.0001.

Article Snippet: Verteporfin, a YAP1 inhibitor, was acquired from Selleck (Selleck, USA).

Techniques: Over Expression, Expressing, Virus, Infection, Control

Journal: Regenerative therapy

Article Title: Transcriptomic analysis of hepatocytes reveals the association between ubiquitin-specific peptidase 1 and yes-associated protein 1 during liver regeneration.

doi: 10.1016/j.reth.2023.07.004

Figure Lengend Snippet: Fig. 6. Potential relationships between USP1 and YAP1 induced hepatocyte proliferation during liver regeneration. PHx: partial hepatectomy, USP1: ubiquitin specific peptidase 1, YAP1: yes-associated protein 1, PCNA: proliferating cell nuclear antigen.

Article Snippet: Verteporfin, a YAP1 inhibitor, was acquired from Selleck (Selleck, USA).

Techniques: Ubiquitin Proteomics